Abstract
The analysis of mycotoxins in food and feed using liquid chromatography coupled with mass spectrometry is considered advantageous because the hyphenated technology enables simultaneous determination of multiple mycotoxins. Multi-mycotoxin analysis requires special consideration of quality control parameters to ensure proper evaluation of data quality for all target mycotoxins in method development and routine sample analysis. Mycotoxin matrix reference materials, especially certified reference materials, are stable and homogeneous matrices with certified traceability, concentrations, and uncertainty for mycotoxin(s) of interest. The use of these reference materials for single mycotoxin analysis has been a well-accepted practice and should be extended to multi-mycotoxin analysis. This opinion piece discusses the following essential metrological and operational components to improve data quality: (1) purposes of multi-mycotoxin reference materials; (2) comparison of reference materials, certified reference materials, and in-house quality control materials; (3) advantages of using reference materials for multi-mycotoxin analysis; (4) current trends and challenges of multi-mycotoxin reference materials. Potential applications of reference materials discussed here can improve routine mycotoxin determination and will lead to better accuracy and consistency of results. Quality control processes that incorporate reference materials in the field of mycotoxin analysis ensure successful development and implementation of liquid chromatography mass spectrometry-based multi-mycotoxin methods.