Abstract
Metastasis is the major cause of breast cancer deaths. To spread from the primary tumor sites to distant tissues, solid tumor cells need to degrade the surrounding extracellular matrix (ECM). The protrusive membrane structures named invadopodia have been shown to play a critical role in the degradation of the ECM and invasion of invasive cancer cells. In this chapter, we describe a detailed protocol to examine invadopodia in human breast cancer cells.