Abstract
Portuguese lavenders remain undervalued in global markets due to the lack of high-quality end-products and scarcity of scientific-based studies validating their bioactive potential. Moreover, chemical variability is frequent in these species, and can compromise both safety and efficacy. In the present study, the anti-inflammatory potential of L. luisieri and L. pedunculata, two highly prevalent species in Portugal, was assessed and correlated with their chemical variability. Representative samples with distinct chemical profiles were selected to assess the anti-inflammatory effect on LPS-stimulated macrophages. L. luisieri essential oil with low quantities of necrodane derivatives was the most potent at inhibiting NO production. Interestingly, the essential oil was more effective than its main compounds (1,8-cineole and fenchone), assessed alone or in combination. Our results also demonstrated a significant effect of the oil on the expression of the inflammatory proteins (iNOS and pro-IL-1β) and on the NF-κB pathway. Overall, this study highlights the impact of chemical variability on oils' efficacy by showing distinct effects among the chemotypes. We also identify L. luisieri essential oil, with low quantities of necrodane derivatives, as the most promising in the mitigation of the inflammatory response, thus corroborating its traditional uses and paving the way for the development of herbal medicinal products.