Sequencing and characterization of striped venus transcriptome expand resources for clam fishery genetics

条纹金星转录组的测序和表征扩展了蛤蜊渔业遗传学资源

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作者:Alessandro Coppe, Stefania Bortoluzzi, Giulia Murari, Ilaria Anna Maria Marino, Lorenzo Zane, Chiara Papetti

Background

The striped venus Chamelea gallina clam fishery is among the oldest and the largest in the Mediterranean Sea, particularly in the inshore waters of northern Adriatic Sea. The high fishing pressure has lead to a strong stock abundance decline, enhanced by several irregular mortality events. The nearly complete lack of molecular characterization limits the available genetic resources for C. gallina. We achieved the first transcriptome of this species with the

Significance

This study represents the first attempt to sequence and de novo annotate the transcriptome of the clam C. gallina. The availability of this transcriptome opens new perspectives in the study of biochemical and physiological role of gene products and their responses to large and small-scale environmental stress in C. gallina, with high throughput experiments such as custom microarray or targeted re-sequencing. Molecular markers, such as the already optimized EST-linked microsatellites and the discovered SNPs will be useful to estimate effects of demographic processes and to detect minute levels of population structuring.

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