Comparison of microarray platforms for measuring differential microRNA expression in paired normal/cancer colon tissues

用于测量配对正常/癌症结肠组织中差异 microRNA 表达的微阵列平台比较

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作者:Maurizio Callari, Matteo Dugo, Valeria Musella, Edoardo Marchesi, Giovanna Chiorino, Maurizia Mello Grand, Marco Alessandro Pierotti, Maria Grazia Daidone, Silvana Canevari, Loris De Cecco

Background

Microarray technology applied to microRNA (miRNA) profiling is a promising tool in many research fields; nevertheless, independent studies characterizing the same pathology have often reported poorly overlapping

Conclusions

Technical and analytical challenges in measuring miRNAs still remain and further research is required in order to increase consistency between different microarray-based methodologies. However, a better inter-platform agreement was found by looking at miRNA sets instead of single miRNAs and through a miRNAs - gene expression integration approach.

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