Abstract
AIM:To compare the effects of liposomes and glyco-poly-L-lysine on liver targeted uptake and expression of plasmid in rat liver.METHODS:After binding with lipofectamine or galactose-terminal glyco-poly-l-lysine, the plasmid could be expressed in eukaryotic cells when injected in to Wistar rats by intravenous route. At different time intervals after the injection, the distribution and expression of the plasmid in liver of rats were observed and compared using in situ hybridization and immunohistochemistry.RESULTS:The expression of the plasmidbinding to liposomes or G-PLL cou ld be markedly observed 24 h later, and began to decrease one week later,but it still could be observed up to three weeks.Both liposomes and G-PLL coul d deliver the plasmid to the liver effectively, but the effect of the latter was better than the former concerning the distribution and expression of the plasmid targeted uptake in the liver.