Abstract
The nitrate reductase S (nirS) type denitrifying bacteria is important for nitrate removal, and suspended particulate matter (SPM) in coastal aquaculture water provides a microenvironment for denitrification to occur. However, it is currently unclear about the denitrification mechanism for nitrate removal occurred on SPM in coastal aquaculture water. SPM with different particle sizes (0.22, 1 and 5 μm) were collected from coastal cage aquaculture ponds water in Maoming City, China. Gene abundance, community structure, diversity and environmental influences of nirS-type denitrifying bacteria on SPM were investigated by fluorescence quantitative PCR (qPCR), high-throughput sequencing and statistical analysis. Roseobacter was widely distributed on SPM with three kinds of particle sizes, especially SPM with 0.22 μm particle size. Pelomonas and Pseudomonas were mainly present on SPM with 1 and 0.22 μm particle sizes, while Acidithiobacillales was mainly present on SPM with 5 μm particle size. The nirS gene abundance and community diversity of denitrifying bacteria was higher on SPM with 5 μm particle size than that with 1 and 0.22 μm particle sizes. The nirS-type denitrifying bacteria communities clustered evidently according to SPM particle sizes in cage aquaculture ponds water. NO(3)(-)-N, NO(2)(-)-N, particle size, and pH were identified as significant factors affecting the community structure and diversity of nirS-type denitrifying keystone genera on SPM. The present study provided new insights into denitrification in the microenvironment on SPM in aquaculture systems, which may contribute to the biological management of cage aquaculture ponds.