Fenlong-ridging combined with composite modifier reconstructs soil microbiome to mitigate saline stress and enhance sustainable cultivation of Isatis indigotica Fortune

芬隆垄沟结合复合改良剂可重建土壤微生物群落,从而缓解盐胁迫并促进菘蓝(Isatis indigotica Fortune)的可持续栽培。

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Abstract

Structural barriers and nutrient scarcity constrain medicinal plant cultivation in coastal saline soils. To address these challenges, we investigated a physical-chemical-microbial synergistic remediation strategy using Fenlong-ridging (FR) and a composite modifier (CM) by evaluating four soil treatments: conventional tillage; FR only; a CM only and FR combined with a CM (FR_CM). A comparative analysis of the four treatments revealed FR_CM to be the most influential strategy for improving coastal saline soil. The FR_CM treatment reduced soil electrical conductivity and bulk density by 78.38% and 29.31%, respectively; enhanced soil nutrient content and Isatis indigotica Fortune biomass by 68.91-114.29% and 126.52%, respectively; induced significant reorganization of microbial community structure at the phylum/OTU levels; elevated microbial network parameters, including positive/negative correlation ratios, modularity, and average path length; and enriched functional taxa such as fermentation and chitinolysis, while enhancing stress-tolerant phenotypes and suppressing potentially pathogenic phenotypes. This work establishes a theoretical foundation and technical paradigm for microbial-mediated remediation in saline-alkali ecosystems to assist crop-to-environment adaptation strategies and maintain sustainable medicinal plant production.

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