Abstract
Industrial microorganisms have a wide range of applications in biomanufacturing, energy production, environmental protectionpharmaceutical development, etc. Synthetic biology has revolutionised the field of industrial microorganisms by designing, constructing and optimising biological systems. The aim of this study is to discuss the key technologies of synthetic biology in industrial microorganisms and their application prospects. Gene editing technology, one of the core tools of synthetic biology, enables researchers to precisely modify microbial genomes to optimise their metabolic pathways or introduce new functions. Metabolic engineering, as an important direction for the application of synthetic biology in industrial microorganisms, enables the efficient synthesis of target products by optimising and reconstructing the metabolic pathways of microorganisms. The development of high-throughput screening and automated platforms has enabled large-scale gene editing and metabolic engineering experiments. The application of synthetic genomics promises to develop microbes with highly customised functions. However, there are still many challenges in this field, and future research still requires interdisciplinary collaboration to drive the application of synthetic biology in industrial microorganisms to new heights.