Abstract
Carbon fixation microorganisms (CFMs) are important components of the soil carbon cycle. However, the global distribution of CFMs and whether they will exceed the environmental tipping points remain unclear. According to the machine learning models, total carbon content, nitrogen fertilizer, and precipitation play dominant roles in CFM abundance. Obvious stimulation and inhibition effects on CFM abundance only happened at low levels of total carbon and precipitation, where the tipping points were 6.1 g·kg(-1) and 22.38 mm, respectively. The abundance of CFMs in response to nitrogen fertilizer changed from positive to negative (tipping point at 9.45 kg ha(-1)·y(-1)). Approximately 46% of CFM abundance decline happened in cropland at 2100. Our work presents the distribution of carbon-fixing microorganisms on a global scale and then points out the sensitive areas with significant abundance changes. The previously described information will provide references for future soil quality prediction and policy decision-making.