Abstract
A study of the inhibitory effect of 24 different combinations of brilliant green and bile salts concentrations was conducted, using seven species of microorganisms capable of fermenting mannitol. The inhibitory effect of brilliant green decreased as the bile salts concentration was increased. Staphylococcus aureus and Proteus rettgeri were inhibited by all test media. Escherichia coli was inhibited in all but two combinations of brilliant green and bile salts. Aerobacter aerogenes generally followed a pattern of growth similar to that of three species of salmonellae. Three of the 24 combinations of brilliant green and bile salts showed little or no inhibition of salmonellae but did inhibit the other organisms studied.