Generation of iPSC lines from primary human chorionic villi cells

Primary human chorionic villi (CV) cells were used to generate the iPSC line by retroviral transduction of the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC. Pluripotency was confirmed both in vivo and in vitro. The transcriptomes of the CV-derived iPSC lines and the human embryonic stem cell lines-H1 and H9 have a Pearson correlation of 0.929 and 0.943 respectively.