Generation of a human fibroblast-derived induced pluripotent stem cell line KRIBBi009-A from a patient with breast cancer

从乳腺癌患者体内生成人类成纤维细胞衍生的诱导性多能干细胞系 KRIBBi009-A

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作者:Naeun Son, Ye Seul Son, Cho-Rok Jung, Mi-Young Son

Abstract

Patient-derived human induced pluripotent stem cells (iPSCs) provide a potentially useful resource for studying disease pathology and therapeutics. In this study, we generated the breast cancer patient-derived KRIBBi009-A-iPSC line from normal fibroblasts using the Sendai virus, which expressed pluripotent markers and exhibited differentiation capacity across 3 germ layers through in vitro differentiation and in vivo teratoma assay. A normal karyotype and the absence of cross-contamination of the cell lines were confirmed. Consequently, the developed iPSC line has been confirmed to be suitable for use in various studies.

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