Abstract
To clarify microbial assembly during saline-alkali wetland degradation, we analyzed bacterial (16S rRNA) and fungal (ITS) communities across four habitats: pristine wetland (PW), transitional meadow wetland (TMW), halophytic herbaceous community (HHC), and converted farmland (CF). Soil water content collapsed from PW (42.22%) to ≤18.40% elsewhere, and soils were alkaline with pH highest in HHC (10.08). Nutrient pools and enzyme activities were highest in PW (SOC 35.03 g kg(-1); URE 142.58 mg g(-1); SUC 527.83 mg g(-1)) but declined sharply under natural degradation, reaching minima in HHC (SOC 8.02 g kg(-1)). ACP and CAT were also lowest in HHC. Bacterial communities were dominated by Actinomycetota and Pseudomonadota, with Acidobacteriota and Bacillota enriched in CF. Bacterial diversity peaked in CF, whereas fungal richness was highest in CF and Shannon diversity peaked in TMW. Ordination and redundancy analyses indicated stronger edaphic control on bacteria than fungi, with pH, SOC, and moisture as key drivers. Null-model analyses showed bacterial assembly shifted toward deterministic selection under saline-alkali stress and agricultural conversion, whereas fungal assembly remained predominantly stochastic. Co-occurrence networks further suggested higher bacterial vulnerability under extreme degradation but comparatively higher fungal robustness. Overall, bacteria and fungi follow divergent assembly rules during saline-alkali wetland degradation.