Abstract
Trehalose, a non-reducing disaccharide (α-D-glucopyranosyl-(1→1)-α-D-glucopyranoside) is a natural compound, which serves as a protective substance in halophilic bacterial cells. Trehalose biosynthesis genes (otsA and otsB) were PCR amplified from the genomic DNA of deep sea actinobacteria, Streptomyces qinglanensis NIOT-DSA03. The amplified genes were cloned and nucleotide sequences were determined. In silico sequence and phylogenetic analysis of nucleotides and amino acids of otsA and otsB sequences of S. qinglanensis were also determined. The experimental data described in this study will be helpful to develop a recombinant expression system to produce trehalose for biotechnological applications.