Abstract
Behavioral, electroencephalographic, and evoked potential studies, as well as experiments utilizing the direct intracerebral injection of benzodiazepines (BZs), have implicated the amygdala, particularly its basolateral nuclear division, as an important locus of the therapeutic actions of these drugs. BZ receptor localization within the amygdala could further define which amygdaloid nuclei are important for BZ action and suggest neuroanatomical pathways especially sensitive to these drugs. Using a quantitative light microscopic autoradiographic technique, this study has localized type 1 and type 2 BZ receptors within the amygdaloid nuclear complex of the rat. BZ receptors were labeled with [3H]flunitrazepam, and type 1 BZ receptor binding was preferentially displaced by CL218,872 in 8-micron coronal sections of rat brain. Autoradiograms were generated by the apposition of emulsion-coated coverslips and quantified by standardized procedures. Ten amygdaloid nuclei were examined at nine levels over the rostrocaudal extent of the amygdala. Total BZ receptor density was highest in the basolateral nuclear complex, and the majority of these receptors were of the type 2 subclass. Type 1 receptors were concentrated in the anterior aspects of the amygdala, particularly the anterior cortical nucleus. These results are consistent with an important role for the basolateral amygdala in anxiogenesis and seizure induction. Furthermore, a larger circuit which includes the amygdala, ventromedial hypothalamus, mammillary body, anterior thalamus, and frontal cortex could be involved in the anxiolytic actions of the BZ drugs.