Abstract
The methylotrophic yeast Komagataella phaffii (formerly known as Pichia pastoris) is an essential host for biotechnological production. Here, we present the complete and annotated genome sequence of CBS 2612 T = NRRL Y-7556T, the type strain of K. phaffii. CBS 2612 has a genome length of 9,387,549 bp with 5412 predicted and 5389 annotated genes, of which 144 are tRNA genes including the previously missing tRNAs for tryptophan, tyrosine, and serine, and 34 rRNA genes. In total, 4 SNPs were found compared to the biotechnologically most commonly used strain, CBS 7435. Additionally, 34 lncRNA candidates could be identified, including candidates that affect telomere-regulation and flocculin genes.