Abstract
SCOBY bacterial cellulose (BC) is a biological macromolecule (considered as a by-product) that grows at the liquid-air interface during kombucha tea fermentation. In this study, BC:HC (hydrolyzed collagen) blend films coated with 1 wt% chitosan (CS) were loaded with ascorbic acid to study loading/releasing performance. At first, the mechanical properties of the blend films were found to be dependent on HC ratio. After chitosan coating, the coated films were stronger due to intermolecular hydrogen bonding interaction and the miscibility of two matrixes at the interface. The antibacterial activity test according to the AATCC Test Method revealed that chitosan-coated BC/HC films exhibited excellent antimicrobial activity against S.aureus growth from the underneath and the above film when compared to BC and BC:HC films. Moreover, chitosan was attractive to ascorbic acid during drug loading. Consequently, its releasing performance was very poor. For BC:HC blend films, ascorbic acid loading/releasing performance was balanced by water swellability, which was controlled using blending formulation and coating. Another advantage of BC films and BC:HC blend films was that they were able to maintain active ascorbic acid for a long period of time, probably due to the presence of plenty of BC hemiacetal reducing ends (protective group).