Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines
改良的 CRISPR/Cas9 介导两种 MKK7 敲除人类胚胎干细胞系的产生
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作者:C H Cole Sims, Matias I Autio, Adriana Buskin, Christine Cheung, Rakesh Heer, Roger S Y Foo, Xin Wang
| 期刊: | Stem Cell Research | 影响因子: | 0.800 |
| 时间: | 2021 | 起止号: | 2021 Apr:52:102238. |
| doi: | 10.1016/j.scr.2021.102238 | 种属: | Human |
| 研究方向: | 信号转导、发育与干细胞 | 细胞类型: | 干细胞 |
Abstract
Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful resource for studying MKK7 function in humans.
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