Abstract
Treadmills are a convenient tool to study animal gait and behavior. Traditional animal treadmill designs often entail preset speeds and therefore have reduced adaptability to animals' dynamic behavior, thus restricting the experimental scope. Fortunately, advancements in computer vision and automation allow circumvention of these limitations. Here, we introduce a series of real-time adaptive treadmill systems utilizing both marker-based visual fiducial systems (colored blocks or AprilTags) and marker-free (pre-trained models) tracking methods powered by advanced computer vision to track experimental animals. We demonstrate their real-time object recognition capabilities in specific tasks by conducting practical tests and highlight the performance of the marker-free method using an object detection machine learning algorithm (FOMO MobileNetV2 network), which shows high robustness and accuracy in detecting a moving rat compared to the marker-based method. The combination of this computer vision system together with treadmill control overcome the issues of traditional treadmills by enabling the adjustment of belt speed and direction based on animal movement.