Abstract
Chromochloris zofingiensis is a green alga that serves as a valuable source of lipids, proteins, and carotenoids. Compared to well-studied microalgal carotenoid producers, C. zofingiensis offers several advantages, including high biomass, lipid and carotenoid productivity as well as less susceptibility to contaminations. C. zofingiensis can achieve growth rates up to four times higher than those of H. pluvialis under optimal phototrophic conditions. Although several studies have examined its cultivation and carotenogenesis under different tropic growth modes at laboratory scale, few have focused on pilot-scale systems. The goal of this study is to investigate the microalga's physiological adaptation in a 200 L tubular photobioreactor during a three-phase semi-continuous cultivation strategy, particularly focusing on the changes in macromolecular and pigment composition. After an initial biomass accumulation phase, a two-phased stress phase was applied combining nutrient depletion (phase 1) and osmotic salt stress conditions (phase 2). Following this procedure, the cellular protein content dropped to 44.7% of its initial level, while the lipid content rose by up to 320%. Additionally, the astaxanthin concentration increased from 1.1 mg/g(DW) to 4.9 mg/g(DW) during the last osmotic stress phases, aligning with results from published laboratory-scale studies.