Abstract
The acidobacterial strain JP12(T) affiliated with the Terriglobales has been isolated from fen soil sampled at Schlöppnerbrunnen II near Bayreuth, Germany. The strain was Gram-stain-negative, non-motile, displayed non-spore-forming rods that divide by binary fission and segregate exopolysaccharide-like structures. JP12(T) grew at temperatures of 4-40 °C (best between 24 and 30 °C), at pH values of 3.7-6.0 (best between 4.1 and 5.6) and at NaCl concentrations of 0-0.5% (best 0.25%, w/v).MK-8 was identified as the major respiratory quinone. The major fatty acids of the strain JP12(T) were iso-C(15:0), C(16:0), iso-C(17:1) ω7c, iso-C(17:0) and iso-diabolic acid. Phosphatidylglycerol, phosphatidylethanolamine and ornithine-containing lipids were the major polar lipids. Lysophosphatidylethanolamine, phosphatidylinositol, diphosphatidylglycerol and high-mass intact polar lipids occurred in small amounts. The G+C content of the strain JP12(T) was 56.4 mol%. 16S rRNA gene sequence similarity values of 96.1-96.7% placed the strain JP12(T) in the vicinity of the type strains of Occallatibacter savannae A2-1c(T), Telmatobacter bradus TPB6017(T), Occallatibacter riparius 277(T) and Terracidiphilus gabretensis S55(T), respectively. Based on the phenotypic, phylogenetic and chemotaxonomic data, we propose the new species Occallatibacter bavaricus sp. nov. (type strain JP12(T)=DSM 110680(T)=CECT 30267(T)) within the acidobacterial order Terriglobales. We also propose the reclassification of Terracidiphilus gabretensis as Occallatibacter gabretensis comb. nov.