Abstract
Production areas influence the quality of highland barley (Hordeum vulgare L.), and fertilization levels may be associated with the origin traceability of highland barley. As the main object of the study, a collection of highland barley was planted in different areas in Tibet, China, to explore the effect of fertilizer on the quality and traceability of highland barley. We carried out field experiments with and without fertilizer treatment (using urea and diamine phosphate). Highland barley was distinguished by nutrient and mineral element contents in combination with chemometric methods. The results indicated that fertilizer treatment significantly affected some mineral element contents in highland barley and improved the accuracy of highland barley traceability. The combination of nutrients and mineral elements could distinguish highland barley from those raised in other areas due to influence of growing environment. P, K, Fe, and Cu provided a great contribution to the classification of highland barley. Thus, the combination of nutrients and mineral elements can be used as a powerful tool to track highland barley, indicating that fertilization treatment should be considered when tracing highland barley.