Abstract
Drought is a major factor limiting crop growth and yield. Enhancing drought resistance is an important strategy to sustain higher yields, with an emphasis on developing drought-tolerant cultivars. In this study, 19 sorghum varieties from both domestic and international sources were selected as experimental materials. At the seedling stage, 11 above-ground and below-ground phenotypic traits were investigated. Under 25% PEG-6000 concentration, drought tolerance during the seedling stage was assessed using differential analysis, such as correlation analysis, principal component analysis (PCA), membership function analysis, regression analysis, and cluster analysis. The present results demonstrate that the principal component analysis could represent 77.18% of the data from the original 11 indicators. Total root length, stem diameter, and leaf area were identified as the main evaluation indicators for sorghum seedling drought tolerance. In addition, based on principal component scores (F) and drought tolerance metric values (D), the 19 sorghum varieties were classified into three categories through systematic cluster analysis: two varieties were classified as highly drought-tolerant, nine as moderately drought-tolerant, and eight as drought-sensitive. Two varieties, such as Longza 24 and Jinza 12, were identified as drought-tolerant during the seedling stage and can serve as valuable resources for evaluating drought tolerance throughout the full growth period and for breeding improvements. Hence, this study established a drought tolerance evaluation method by integrating above- and below-ground phenotypic traits indicators, providing theoretical support for the identification of drought-tolerant sorghum.