Abstract
This study focuses on multiple origins of green-back purple and dual-faced purple Perilla frutescens, employing field cultivation experiments combined with detection methods, such as HPLC, LC-MS, and GC-MS, to compare the differences in yield, quality, and metabolic products of the different colored P. frutescens. The results indicate that green-back purple P. frutescens significantly outperformed dual-faced purple P. frutescens in terms of leaf, stem, and seed yields, while the effective component contents in the leaves and seeds of dual-faced purple P. frutescens are higher than those of dual-faced green P. frutescens. An analysis of the anthocyanin components in P. frutescens leaves and the volatile components in P. frutescens seeds shows that the total anthocyanin content in dual-faced purple P. frutescens leaves is 34.63% higher than that in green-back purple P. frutescens, whereas the total volatile components in the seeds of green-back purple P. frutescens exceeds those in dual-faced P. frutescens by 12.99%. The Mantel test indicates a potential correlation mechanism between the anthocyanin components in P. frutescens leaves and the volatile components in P. frutescens seeds, which are significantly associated with the yield quality of both P. frutescens leaves and seeds. This study found that P. frutescens with blue-green leaves yields more than double-sided purple P. frutescens, although the quality of its leaves and seeds is inferior to that of double-sided purple P. frutescens. Furthermore, the anthocyanin components in P. frutescens leaves and the volatile components in P. frutescens seeds exhibit significant correlations with the yield and quality of both leaves and seeds, offering important insights for the production and application of P. frutescens.