Abstract
The NAC (NAM, ATAF1/2, and CUC2) family is one of the largest plant-specific transcription factor families, playing a crucial role in adaptation to abiotic stresses. However, the NAC gene family in sweet cherry (Prunus avium L.) remains poorly understood. In this study, we identified 130 NAC genes (PaNAC) from the sweet cherry genome, which were unevenly distributed across eight chromosomes. Phylogenetic analysis classified the PaNACs into 21 distinct groups, including 2 sweet cherry-specific groups. Comparative analysis revealed significant variations in gene proportions, exon-intron structures, and motif compositions among different groups. Furthermore, cis-element analysis suggested the potential roles of PaNACs in regulating plant growth, development, hormone signaling, and stress responses. Transcriptomic data revealed tissue-specific expression patterns for several PaNAC genes. qRT-PCR further confirmed that eight selected PaNACs were responsive to various abiotic stresses in Gisela 6, a widely used hybrid rootstock in sweet cherry production that shares high sequence similarity in NAC genes with P. avium. These findings provide valuable insights for future research on the functional characteristics of the PaNAC genes in the growth, development, and responses to abiotic stress in sweet cherry.