Abstract
In this study, the gene ZmPR5, associated with resistance to ear rot, was identified through transcriptome data analysis of the maize inbred line J1259. The gene was subsequently cloned and its function was investigated. The ZmPR5 gene comprises an open reading frame of 525 base pairs, encoding a protein of 175 amino acids. ZmPR5 was overexpressed in Arabidopsis and the ZmPR5EMS mutant in maize, and they were subjected to q-PCR and measurements of antioxidant enzyme activities (POD, SOD, CAT, MDA), electrical conductivity, and chlorophyll content. The results indicate that the expression of ZmPR5 is up-regulated upon infection by Fusarium verticillioides, with significant differences observed in the activities of POD, SOD, CAT, MDA, electrical conductivity, and chlorophyll content. The study found that ZmPR5 is localized in the nucleus and interacts with Zm00001d020492 (WRKY53) and Zm00001d042140 (glucA). Trypan blue staining revealed that the stained area in the ZmPR5EMS mutant of maize was significantly larger than that in B73. The expression of ZmPR5 is closely associated with resistance to maize ear rot.