Metabolic and Photosynthesis Analysis of Compound-Material-Mediated Saline and Alkaline Stress Tolerance in Cotton Leaves

化合物介导的棉花叶片耐盐碱胁迫的代谢和光合作用分析

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Abstract

Soil salinization and alkalization can cause great losses to agricultural production in arid regions. Cotton, a common crop in arid and semi-arid regions in China, often encounters saline stress and alkaline stress. In this study, NaCl (8 g·kg(-1)), Na(2)CO(3) (8 g·kg(-1)), and a compound material (an organic polymer compound material) were mixed with field soil before cotton sowing, and the ion content, photosynthetic characteristics, and metabolite levels of the new cotton leaves were analyzed at the flowering and boll-forming stage, aiming to clarify the photosynthetic and metabolic mechanisms by which compound material regulates cotton's tolerance to saline stress and alkaline stress. The results showed that the application of the compound material led to an increase in the K(+)/Na(+) ratio, stomatal conductance (Gs), efficiency of PSII photochemistry (ψPSⅡ), potential activity (Fv/Fo), and chlorophyll content (Chla and Chlb), as well as the abundances of D-xylonic acid and DL-phenylalanine in the NaCl treatments. Additionally, there were increases in the K(+) content, K(+)/Na(+) ratio, Chla/b ratio, net photosynthetic rate (Pn), transpiration rate (Tr), ψPSⅡ, and D-saccharic acid abundance in the Na(2)CO(3) treatments. A correlation analysis and a metabolic pathway analysis revealed that the compound material mainly regulated the photosynthetic characteristics of and the ion balance in the new leaves through regulating the abundance of key metabolites when the cotton was under NaCl stress or Na(2)CO(3) stress. Furthermore, the positive impact of the compound material on the cotton's NaCl stress tolerance was stronger than that on the cotton's Na(2)CO(3) stress tolerance.

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