Abstract
The Asteraceae family is a large plant family, with over 1600 genera and 25,000 species, most of which are generally herbaceous plants. This family's members are widely used in the human diet and medicine. One of the most popular representatives is Centaurea benedicta L., known as 'Blessed Thistle'. It is a famous plant in the herbal world with some medical benefits, such as strong antioxidant and antidepressant effects, with antibacterial and antiseptic properties, a stimulant of appetite, with a good effect on the liver and the secretion of bile juices, etc. Therefore, this work aimed to fully characterize the chemical composition of the seeds of C. benedicta introduced in Bulgaria, some of the physicochemical characteristics, as well as the biologically active compounds. The main nutrient in the chemical composition was carbohydrates (68.5%), and half of their quantity was occupied by fibers (32.2%). Total proteins accounted for 16.4%, and the glyceride oil content was rather low-about 11.0%. The main fatty acids identified in the seed oil were linoleic (72.1%) and oleic acids (18.1%), and the amount of the polyunsaturated ones predominated (73.0%). The main lipid-soluble bioactive components were sterols (0.9%), phospholipids (1.9%), and tocopherols (492 mg/kg). β-Sitosterol (59.5%) and stigmasterol (19.4%) were the main sterols, and α-tocopherol (472 mg/kg) predominated in the tocopherol fraction. The major phospholipids were phosphatidylethanolamine (45.4%), followed by phosphatidylinositol (37.1%) and phosphatidylcholine (6.1%).