Abstract
The antifungal and antioxidant properties of essential oils (EOs) derived from four plants were assessed in vitro: Rosmarinus officinalis, Myrtus communis, Origanum compactum, and Eugenia aromatica. These plants are renowned for their diverse biological activities. Antioxidant activities were evaluated using DPPH, ABTS, and TAC tests. Antifungal activity was tested against four postharvest pathogens associated with chickpea in storage: Fusarium culmorum, Rhizopus oryzae, Penicillium italicum, and Aspergillus niger, using the broth microdilution technique. Additionally, the efficacy of several major compounds against fungi found in the EOs 1,8-cineole, carvacrol, and eugenol was evaluated. Furthermore, this study explored the potential synergy of combining eugenol and carvacrol in various ratios. Based on the results, E. aromatica EO exhibited the highest antioxidant activity, as evidenced by its lowest IC(50) values for a DPPH of 0.006 mg/mL. This EO also demonstrated the best antifungal activity, with MIC values ranging from 0.098 to 0.13 μL/mL. The high concentration of eugenol in this oil was identified as a contributing factor to its potent antifungal effects. The individual application of eugenol displayed significant antifungal efficacy, which was further enhanced by incorporating carvacrol at a 1:3 ratio. This synergistic combination presents promising potential for the development of specific formulations aimed at optimizing grain protection during storage.