Multivariate analysis of FT-IR, bioactive compounds, and antioxidant activity in coffee pulp (Coffea arabica var. Catimor): combined effects of ripening stage and drying method

咖啡果肉(阿拉比卡咖啡,品种:卡蒂莫)中FT-IR光谱、生物活性化合物和抗氧化活性的多变量分析:成熟阶段和干燥方法的综合影响

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Abstract

Coffee pulp, a nutrient-rich by-product, holds untapped nutraceutical potential but requires postharvest optimization. This study evaluated how ripening stage and drying method jointly modulate bioactive compounds in Coffea arabica var. Catimor pulp. FT-IR spectroscopy, HPLC-DAD, and antioxidant assays, coupled with multivariate analysis, revealed drying as the primary driver of compositional changes. FT-IR PCA (97.5 % variance in PC1) distinguished freeze-dried from thermally treated samples, while key VIP bands associated with lignin and structural carbohydrates, together with bioactive profiles (chlorogenic acid, ABTS, caffeine), revealed thermal degradation as a unifying process shaped by ripening stage. Drying × ripening effects modulated all variables except caffeine, which declined with ripening. Thermal treatments reduced phenolics, DPPH, trigonelline, and 5-CQA, with greater losses in overripe pulp. ABTS increased with roasting, while peak levels occurred in ripe freeze-dried samples. These results underscore the need for ripening-specific drying protocols to optimize coffee pulp's functional quality.

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