Abstract
As the global demand for sustainable protein sources grows, valorizing side streams in plant protein processing has become crucial. This study revisits the conventional alkaline-isoelectric extraction of faba bean protein isolates, introducing an enhanced mass balance-driven approach to recover underutilized protein fractions from typically discarded side streams. Through strategic pH manipulation and centrifugation, four distinct protein fractions were recovered with purities ranging from 34.6% to 89.6%, collectively recapturing a significant portion of the 16% protein loss in standard processing. SDS-PAGE and FTIR analyses confirmed the structural diversity among the recovered fractions, with albumin-rich and globulin-rich profiles exhibiting unique spectral and electrophoretic signatures. Functionally, fractions B and D exhibited superior water- and oil-holding capacities, indicating their potential utility in food formulations requiring enhanced moisture and lipid retention. In contrast, fraction C, characterized by low water-holding capacity and high solubility, may be better suited to applications prioritizing emulsification performance, such as in dairy or meat analogs. This study not only highlights the feasibility of reclaiming high-quality protein from industrial byproducts but also underscores the potential of these recovered proteins in diverse food and non-food sectors, including pharmaceuticals and cosmetics. These findings contribute to circular economy strategies by transforming waste into value-added ingredients with functional and commercial significance.