Abstract
Date seeds, often discarded during industrial processing, are an underexploited by-product rich in polyphenols with significant antioxidant potential. This study explores the extraction of polyphenols from date seeds using microwave-assisted extraction (MAE) with an organic solvent. The extraction process was optimized using response surface methodology (RSM), focusing on extraction time, ethanol concentration, and temperature. The optimal extraction conditions were 46% (v/v) of ethanol, at 62 °C and for 27.3 min. Under these optimized conditions, the extraction yield and total phenolic content of the extract are 12.5% and 59 mg gallic acid equivalent g(-1) of date seed, respectively, as confirmed by the experimental tests. The extract's antioxidant activity was confirmed through DPPH, ABTS, and FRAP assays. High-performance liquid chromatography with diode-array detection (HPLC-DAD) identified major phenolic compounds, including procyanidin B1, catechin, quercetin-3,5'-di-O-glucoside, epicatechin, procyanidin B, and syringic acid, alongside eight other tentatively identified compounds. These findings underscore the potential of MAE as an environmentally friendly technique for producing polyphenol-rich extracts from date seeds, adding value to this by-product and opening avenues for its application in food and nutritional products.