Abstract
Functional foods incorporating probiotics, essential oils, and DHA, PhosphatidylSerine have been explored as innovative approaches to improving oral health. This study evaluates the effects of consuming two versus five HETAFU candies daily on oral microbial levels over eight weeks. To compare the impact of moderate (two candies/day) and higher (five candies/day) HETAFU candy consumption on oral microbial populations, focusing on Streptococcus mutans, Lactobacillus, Actinomycetes, and Candida. A total of 200 participants were randomly divided into two groups: Group 1 consumed two candies daily, and Group 2 consumed five candies daily. Microbial levels were measured at baseline, 2 weeks, 4 weeks, and 8 weeks using microbiological techniques. Statistical analysis included Z scores and P values to determine the significance of microbial changes between groups. Both groups showed significant reductions in Streptococcus mutans and Candida, with a concurrent decrease in Lactobacillus and Actinomycetes levels over time. However, Group 2 demonstrated greater microbial shifts, indicating enhanced modulation of the oral microbiome with higher candy consumption. Higher consumption of HETAFU candies (five/day) provides superior benefits in reducing pathogenic microbes and promoting beneficial bacteria compared to moderate consumption (two/day). This suggests a dose-dependent relationship, with increased intake offering additional protective effects against oral microbial imbalances. Functional foods like HETAFU candies represent a promising adjunct to oral hygiene practices.