Abstract
This study aimed to evaluate the anti-obesity effect of Bifidobacterium adolescentis SPM2022 (SPM2022). Culture supernatant of SPM2022 reduced lipid accumulation (p < 0.01) and expression of adipogenic genes (p < 0.001) during 3T3-L1 differentiation. The effects on anti-obesity and lipid metabolism were assessed in the high fat diet (HFD)-induced obese C57BL6 mouse after 4 weeks of obese induction. Experimental groups (n = 6/group): normal diet, HFD, and HFD fed with 10(9) CFU/day of SPM2022. After 5 weeks, the weight gain of SPM2022 group was reduced by 72% and the weight of mesenteric fat was reduced by 60% in the SPM2022 compared to the HFD group. The expression of FABP4, CD36 and leptin in epididymis were decreased to 4.5, 2 and twofold respectively in the SPM2022 compared to HFD group. Based on these results, SPM2022 has the potential to be used as a therapeutic agent for addressing obesity and lipid metabolism. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10068-025-02013-8.