Abstract
Hydrogen sulfide (H&sub2;S) is produced throughout the gastrointestinal tract, and it contributes to maintenance of mucosal integrity, resolution of inflammation, and repair of damaged tissue. H&sub2;S synthesis is elevated in inflamed and damaged colonic tissue, but the enzymatic sources of that synthesis are not completely understood. In the present study, the contributions of three enzymatic pathways to colonic H&sub2;S synthesis were determined, with tissues taken from healthy rats and rats with colitis. The ability of the colonic tissue to inactivate H&sub2;S was also determined. Colonic tissue from rats with hapten-induced colitis produced significantly more H&sub2;S than tissue from healthy controls. The largest source of the H&sub2;S synthesis was the pathway involving cysteine amino transferase and 3-mercaptopyruvate sulfurtransferase (an α-ketoglutarate-dependent pathway). Elevated H&sub2;S synthesis occurred specifically at sites of mucosal ulceration, and was not related to the extent of granulocyte infiltration into the tissue. Inactivation of H&sub2;S by colonic tissue occurred rapidly, and was significantly reduced at sites of mucosal ulceration. This correlated with a marked decrease in the expression of sulfide quinone reductase in these regions. Together, the increased production and decreased inactivation of H&sub2;S at sites of mucosal ulceration would result in higher H&sub2;S levels at these sites, which promotes of resolution of inflammation and repair of damaged tissue.