Abstract
In this study, C. pyrenoidosa were cultured with seven different concentrations of Na(2)SeO(4) (0-10 mg/L), and the effects of Na(2)SeO(4) on the growth, Se-forms, and nutritional quality of C. pyrenoidosa were explored. The results showed that at the concentration of 0.5 mg/L Na(2)SeO(4), the C. pyrenoidosa were plump and healthy; the contents of biomass, soluble protein, lipids, and TPUFA reached the highest level; the total Se content in C. pyrenoidosa increased with the increasing Na(2)SeO(4) concentrations. However, the proportion of organic Se in C. pyrenoidosa. reached the highest value of 87.58% at the concentration of 0.5 mg/L Na(2)SeO(4). Among organic Se forms, SeMet accounted for the largest proportion, while MeSeCys accounted for a relatively smaller proportion, but SeCys(2) was not detected. The addition of Na(2)SeO(4) (except for ≤0.5 mg/L) reduced the contents of photosynthetic pigments in C. pyrenoidosa. In addition, the antioxidant capacity of C. pyrenoidosa first increased and then decreased with the increasing Na(2)SeO(4) concentrations, but different enzymes exhibited different tolerances to Na(2)SeO(4). Based on the above research results, 0.5 mg/L Na(2)SeO(4) concentration is recommended for the production of Se-rich C. pyrenoidosa. Our findings will provide a theoretical basis and practical references for the development of Se-rich C. pyrenoidosa health care products.