Abstract
In horses, surgical site infections (SSIs) are multifactorial complications influenced by patient-related, procedural, and environmental factors. While perioperative contamination has been extensively studied in the operating theater, the anesthetic induction and recovery environment has received limited attention. The aim of this study was to characterize bacterial contamination within an equine anesthetic induction and recovery stall and to evaluate its spatial and temporal distribution. Environmental samples were collected from four predefined locations within the stall at three time points: before patient admission, after recovery from general anesthesia, and after routine cleaning and disinfection. Samples were analyzed using standard aerobic culture techniques, and bacterial species were identified. Bacterial contamination was detected at all sampling locations and time points. Contamination increased significantly after recovery from general anesthesia and was most pronounced in areas associated with moisture retention, such as the groove between floor tiles. The bacterial species identified were predominantly opportunistic environmental organisms. No direct association between environmental contamination and surgical site infections could be established. However, the constant presence of potentially pathogenic bacteria indicates that the anesthetic induction and recovery stall represents a relevant component of the perioperative environment. These findings support the inclusion of the recovery area in infection control strategies aimed at reducing perioperative risk in equine surgical patients.