Abstract
Colorectal cancer (CRC) is a common malignant tumor occurring in the colon. It has been known that the gut microbiota is a complex ecosystem and plays an important role in the pathogenesis of colorectal cancer. Our previous study showed that bound polyphenol of the inner shell (BPIS) from foxtail millet bran exhibited significant antitumor activities in cancer cells and nude mice models. In the present study, the anticancer potential of BPIS is evaluated in the azoxymethane (AOM) and dextran sodium sulfate (DSS)-induced mouse CRC model. Results showed that BPIS could decrease the number and volume of tumors and protect the epithelial architecture from damage. Certain biomarkers associated with CRC formation, such as COX-2, EMR1, PCNA, and caspase-3, were strongly changed by BPIS. Moreover, by 16S rRNA gene sequence analysis, it was found that BPIS could remodel the overall structure of the gut microbiota from tumor-bearing mice toward that of the normal counterparts, including two phyla and eight genera, together with regulations on several genes that are responsible for 17 signaling pathways.