Abstract
Probiotic-based therapies have great potential to treat inflammatory bowel disease (IBD). Still, their success is limited by difficulties in ensuring survival, colonization, and targeted effects within the harsh environment of the gastrointestinal tract. This study presents a bio-coated probiotic strategy for treating colitis in mice, using self-crosslinking mussel adhesive protein (Map) to form a protective layer around Bifidobacterium longum (BL). Because Map contains abundant lysine residues that confer positive charges, it interacts electrostatically with negatively charged alginate (Alg) to form the BL@Map@Alg formulation. Compared to uncoated BL, BL@Map@Alg increased survival under oxygen exposure by 19.6-fold, showed a 76.8-fold increase in resistance to simulated stomach acid, and exhibited a 6.2-fold boost in intestinal colonization. It also demonstrated superior ROS scavenging ability, decreasing ROS levels by 41.1 %. In a mouse colitis model, BL@Map@Alg reduced weight loss, colon shortening, and inflammatory cytokines compared to untreated colitis. It also helped restored intestinal barrier function by increasing the levels of tight junction proteins ZO-1 and claudin-1, which were diminished in DSS-treated mice. 16S rRNA sequencing showed that BL@Map@Alg lowered Escherichia abundance from 27.5 % to 0.042 % while increasing Akkermansia from 0.73 % to 9.3 %. These results highlight the potential for BL@Map@Alg to enhance probiotic therapy by improving survival, colonization, and gut microbiota, offering a promising strategy for personalized and precision medicine.