BactoRamanBioNet: A Multimodal Neural Network for Bacterial Species Identification Using Raman Spectroscopy and Biological Knowledge

BactoRamanBioNet:一种利用拉曼光谱和生物学知识进行细菌物种识别的多模态神经网络

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Abstract

Accurate and rapid identification of bacterial species is essential for public health, clinical diagnostics, and environmental monitoring. Although Raman spectroscopy offers a powerful, non-invasive alternative, reliance solely on spectral data often fails to distinguish species with highly similar signatures, particularly when the discriminating features are subtle. This difficulty is frequently compounded by a lack of integrated biological prior knowledge, which can hinder model performance. To address these challenges, we introduce BactoRamanBioNet, a novel multimodal neural network architecture. Our model employs a synergistic approach that utilizes a ResNet-Transformer architecture to capture complex spectral patterns and a CLIP text encoder to incorporate descriptive biological information, thereby enabling highly accurate multimodal classification of bacterial species. Empirical results demonstrate that BactoRamanBioNet achieves a classification accuracy of 98.2% and an F1-score of 98.0%. This performance surpasses the current state-of-the-art deep learning model, ResNet-1D, by 2.4% in accuracy and 2.0% in F1-score. Moreover, our model outperforms traditional classifiers, such as Support Vector Machine (SVM) and Random Forest (RF), by 9.8% and 7.9% in accuracy, respectively, while also exhibiting significant improvements in precision and recall. By establishing a new benchmark in performance and robustness, BactoRamanBioNet offers a powerful and reliable framework for automated microbiological analysis, paving the way for next-generation diagnostic systems.

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