Generation of a male common marmoset embryonic stem cell line DSY127-BV8VT1 carrying double reporters specific for the germ cell linage using the CRISPR-Cas9 and PiggyBac transposase systems

使用 CRISPR-Cas9 和 PiggyBac 转座酶系统生成携带针对生殖细胞谱系的双报告基因的雄性普通狨猴胚胎干细胞系 DSY127-BV8VT1

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作者:Sho Yoshimatsu, Tsukika Sato, Masafumi Yamamoto, Erika Sasaki, Mayutaka Nakajima, Mari Nakamura, Seiji Shiozawa, Toshiaki Noce, Hideyuki Okano

Abstract

BLIMP1 (PRDM1) and VASA (DDX4) play pivotal roles in the development of the germ cell linage. Importantly, these genes are specifically expressed in germ cells; BLIMP1 in primordial germ cells (PGCs) to early-stage gonocytes, and VASA in migration-stage PGCs to mature gametes. The high reproductive efficiency of common marmosets (marmosets; Callithrix jacchus) makes them advantageous for use in germ cell research. We herein report the generation of a male marmoset embryonic stem cell (ESC) line harboring BLIMP1 and DDX4 double reporters. This ESC line will be a useful tool for investigating male gametogenesis in non-human primates.

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