Abstract
Microalgal biotechnology holds the potential for renewable biofuels, bioproducts, and carbon capture applications due to unparalleled photosynthetic efficiency and diversity. Outdoor open raceway pond (ORP) cultivation enables utilization of sunlight and atmospheric carbon dioxide to drive microalgal biomass synthesis for production of bioproducts including biofuels; however, environmental conditions are highly dynamic and fluctuate both diurnally and seasonally, making ORP productivity prediction challenging without time-intensive physical measurements and location-specific calibrations. Here, for the first time, we present an image-based deep learning method for the prediction of ORP productivity. Our method is based on parameter profile plot images of sensor parameters, including pH, dissolved oxygen, temperature, photosynthetically active radiation, and total dissolved solids. These parameters can be remotely monitored without physical interaction with ORPs. We apply the model to data we generated during the Unified Field Studies of the Algae Testbed Public-Private-Partnership (ATP(3) UFS), the largest publicly available ORP data set to date, which includes millions of sensor records and 598 productivities from 32 ORPs operated in 5 states in the United States. We demonstrate that this approach significantly outperforms an average value based traditional machine learning method (R(2) = 0.77 ≫ R(2) = 0.39) without considering bioprocess parameters (e.g., biomass density, hydraulic retention time, and nutrient concentrations). We then evaluate the sensitivity of image and monitoring data resolutions and input parameter variations. Our results demonstrate ORP productivity can be effectively predicted from remote monitoring data, providing an inexpensive tool for microalgal production and operational forecasting.