Abstract
Bee pollen is a nutrient-rich bee product and natural food supplement that contains proteins, vitamins, minerals and bioactive compounds, offering antioxidant, anti-inflammatory, immunostimulatory and antimicrobial activity. Numerous studies have confirmed the in vitro antimicrobial activity of both polyfloral and monofloral bee pollen. Monofloral bee pollen had a more stable chemical composition and more consistent sensory and biochemical properties, making it more suitable for various applications. This has led to a growing number of studies investigating its antimicrobial potential. Antimicrobial activity of bee pollen is influenced by natural factors such as the botanical and geographical origin, seasonal variation and beekeeping practices. The outcomes of in vitro testing also depend on choices related to extract preparation, solvent type, microbial strains and the method employed to measure antimicrobial activity. Another challenge is the limited bioavailability of bioactive compounds, restricted by the degradation-resistant outer layer of bee pollen, named the exine. The wall can be partially disrupted through processing methods that break it and enhance its nutritional and functional properties. This review provides a comprehensive overview of published studies on the antimicrobial activity of monofloral bee pollen. It summarizes the most frequently investigated botanical species and bacterial strains, highlighting those with the most promising antimicrobial results. Additionally, it examines the processing methods of pollen, comparing their effectiveness and the changes in antimicrobial activity before and after processing. The review identifies the plant species, solvents and methods that yield strong antimicrobial activity, emphasizing their potential in the broader effort to standardize high quality parameters for bee pollen.