Abstract
Microsampling is a technique involving the collection of a small amount of biological material in a volume of several to dozens of microliters. The most common material collected is blood from a fingertip. Following a finger prick, dry blood spots are formed after being transferred to a carrier. The method is microinvasive and causes little discomfort during the collection. The preservation of blood in the form of dry spots seems to be an ideal tool for pesticide exposure assessment in the general population and occupationally exposed groups. Populations in agricultural areas are constantly exposed to environmental xenobiotics, including a wide range of pesticides and other pollutants. This technique can be a practical way to assess pesticide exposure, particularly in rural areas or places where whole blood collection is difficult. The issue has generated considerable scientific debate, primarily due to challenges associated with the analysis of small blood spots of unknown volume and the influence of the haematocrit (HCT) effect. The use of dry blood spots can significantly contribute to the expansion of the scale of pesticide biomonitoring, which will allow for the quick and effective identification of individuals exposed to their effects. The technique of microsampling and analysis of DBS indicate great potential in the assessment of pesticide exposure due to its simplicity of specimen collection, minimal invasiveness, and the possibility of storing and transporting the material without the use of specialized conditions. Clinical trial number: not applicable.