Abstract
To address the growing challenge of antibiotic resistance, magnetic nanoparticles were developed and characterized by providing an innovative solution. Zinc ferrite nanoparticles (ZnFe₂O₄ NPs) were synthesized using a chemical co-precipitation method, stabilized with citric acid (CA), and conjugated with amoxicillin (AX) to create ZnF-CA-AX nanocomposites. These nanoparticles were extensively characterized by their structural and optical properties. The antimicrobial activity of the nanocomposites was tested against gram-positive Staphylococcus aureus and gram-negative Escherichia coli, showing significant inhibition zones. Furthermore, the nanocomposites showed a high level of antibiofilm efficiency, and potential antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl. Collectively, these findings indicate that magnetic nanoparticles can enhance antibiotic effectiveness, offering new therapeutic avenues to combat resistant bacterial infections.