Abstract
Orchard fruits such as pear and apple are important for ensuring global food security and agricultural economy as they not only provide essential nutrients, but also support biodiversity and ecosystem services. Breeders, growers and plant researchers constantly study desirable tree morphological features and floral characteristics to ensure fruit production and quality. Still, traditional orchard phenotyping is often laborious, limited in scale and prone-to-error, resulting in many attempts to develop reliable and scalable toolkits to address this challenge. Here, we present OrchardQuant-3D, an analytic pipeline for automating tree-level analysis of key canopy and floral traits for different types of fruit orchards. We first built a data fusion algorithm to register 3D point clouds collected by both drones (for colour signals) and Light Detection And Ranging (LiDAR, for precise spatial properties), reconstructing high-quality 3D orchard models at different growth stages. Then, we utilised precise global navigation satellite system signals to position trees in orchards with millimetre-level accuracy, enabling tree-level analysis of key canopy (e.g. crown volume and the number or branches) and floral traits (e.g. blossom clusters and volumes) using 3D computer vision, complex graph theory and feature engineering techniques. Equipped with the OrchardQuant-3D pipeline, we successfully measured varietal differences of four pear cultivars from a small pear orchard in Nanjing China, followed by a scale-up study that surveyed 3D tree morphologies, key floral and fruit traits from 1104 apple trees in an orchard in East Malling, United Kingdom. To the best of our knowledge, such a multi-source, comprehensive and expandable methodology has not yet been introduced to this important research domain. Hence, we believe that our work demonstrates a step change in our ability to conduct scalable 3D orchard phenotyping, which is highly valuable to advance orchard breeding, precise tree management and orchard research greatly to sustain fruit tree production in a rapidly changing climate.