Abstract
Higher bone mineral density (BMD) is often associated with greater consumption of black tea (BT). However, the dose-response of BT on mineralization in an osteoblast cell model has not yet been studied. The study objective was to determine the dose-dependent response of BT in Saos-2 cells and investigate changes to several proteins involved in the mineralization process. Mineralization was induced in the presence of BT at concentrations that represent levels likely achieved through daily consumption (0.1, 0.5, 0.75, 1 μg gallic acid equivalents [GAE]/mL) or through supplementation (2, 5, or 10 μg GAE/mL). BT exerted a positive dose-response on bone mineralization, peaking at 1 μg GAE/mL of BT (P < .05). Cellular activity was significantly greater than control with exposure to 2-10 μg GAE/mL of BT (at 24 h) (P < .05) and 1-10 μg GAE/mL (at 48 h) (P < .05), with a peak at 5 μg GAE/mL at 24 and 48 h (P < .05). Protein expression of alkaline phosphatase and ectonucleotide pyrophosphatase/phosphodiesterase-1 were unchanged, whereas a moderate dose of BT (0.75 μg GAE/mL) resulted in greater expression of osteopontin compared with the highest dose (10 μg GAE/mL) (P < .05). Doses of BT from 0.5 to 10 μg GAE/mL resulted in higher antioxidant capacity compared with control (P < .05). In summary, the higher antioxidant capacity, enhanced cell viability, and upregulated mineralization suggest that consumption of BT may have a positive effect on BMD at levels obtained through consumption of tea.