Abstract
Fifteen Gram-stain-negative, rod-shaped bacterial strains, motile by means of a monopolar flagellum, were isolated from glaciers located on the Tibetan Plateau, P.R. China. These strains, designated MDT1-19(T), MDB2-8(T), HLS12-2(T), HLX7-2(T), LB2P49(T), LB2P70(T), LB3P112(T), LB3P118(T), LB2P10(T), RB2R34(T), ZB1P44(T), RB2P8(T), GB1R12(T), GB4P2(T) and RT4P48(T), belong to the genus Janthinobacterium based on 16S rRNA gene sequences analysis. The average nucleotide identity and digital DNA-DNA hybridization values between these strains and species with validly published names were below 96% and 70%, respectively. Based on the phylogenetic, genotypic and phenotypic evidence, these 15 strains are proposed to represent fifteen novel species within the genus Janthinobacterium, with the following proposed names: Janthinobacterium algoris sp. nov. (MDT1-19(T)=CGMCC 1.9797=NBRC 116353(T)), Janthinobacterium breve sp. nov. (MDB2-8(T)=CGMCC 1.9853(T)=NBRC 116354(T)), Janthinobacterium darwini sp. nov. (HLS12-2(T)=CGMCC 1.9980(T)=NBRC 116355(T)), Janthinobacterium woesei sp. nov. (HLX7-2(T)=CGMCC 1.9990(T)=NBRC 116356(T)), Janthinobacterium salmonicoloratum sp. nov. (LB2P49(T)=CGMCC 1.11246(T)=NBRC 116357(T)), Janthinobacterium violaceum sp. nov. (LB2P70(T)=CGMCC 1.11252(T)=NBRC 116358(T)), Janthinobacterium leeuwenhoeki sp. nov. (LB3P112(T)=CGMCC 1.11294(T)=NBRC 116359(T)), Janthinobacterium pasteuri sp. nov. (LB3P118(T)=CGMCC 1.11299(T)=NBRC 116360(T)), Janthinobacterium paucivorans sp. nov. (LB2P10(T)=CGMCC 1.11332(T)=NBRC 116361(T)), Janthinobacterium longum sp. nov. (RB2R34(T)=CGMCC 1.11894(T)=NBRC 116362(T)), Janthinobacterium glycogeni sp. nov. (ZB1P44(T)=CGMCC 1.23234(T)=NBRC 116363(T)), Janthinobacterium kochi sp. nov. (RB2P8(T)=CGMCC 1.23776(T)=NBRC 116364(T)), Janthinobacterium goodfellowi sp. nov. (GB1R12(T)=CGMCC 1.24272(T)=NBRC 116365(T)), Janthinobacterium lyxosi sp. nov. (GB4P2(T)=CGMCC 1.24307(T)=NBRC 116366(T)) and Janthinobacterium amylolyticum sp. nov. (RT4P48(T)=CGMCC 1.24355(T)=NBRC 116367(T)).