Abstract
Increasing demand for green or biological nanoparticles has led to various green technologies and resources, which play a critical role in forming biocompatible or green nanoparticles. So far, numerous medicinal plants have been explored for this purpose, assuming that medicinal components from the plant's material will contribute to corona formation around nanoparticles and enhance their efficacy. Research is also extended to other green and waste resources to be utilized for this purpose. In the current study, we explored Ligustrum vulgare berries, also known as privet berries, to reduce gold and silver salts into nanoparticles. L. vulgare berries showed great potential to form these nanoparticles, as gold nanoparticles (LV-AuNPs) formed within 5 min at room temperature, and silver nanoparticles (LV-AgNPs) formed in 15 min at 90 °C. LV-AuNPs and LV-AgNPs were characterized by various analytical methods, including UV-Vis, SEM, EDX, TEM, DLS, sp-ICP-MS, TGA, FT-IR, and MALDI-TOF. The results demonstrate that the LV-AuNPs are polydisperse in appearance with a size range 50-200 nm. LV-AuNPs exhibit various shapes, including spherical, triangular, hexagonal, rod, cuboid, etc. In contrast, LV-AgNPs are quite monodisperse, 20-70 nm, and most of the population was spherical. The nanoparticles remain stable over long periods and exhibit high negative zeta potential values. The antimicrobial investigation of LV-AgNPs demonstrated that the nanoparticles exhibit antibacterial ability with an MBC value of 150 g/mL against P. aeruginosa and 100 g/mL against E. coli, as determined by plate assay, live and dead staining, and SEM cell morphology analysis.